5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193)
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase (NAD(P)H) is part eukaryotic version of riboflavin biosynthesis


Glyceraldehyde-3-P
|
| (unidentified) (PMID 17014089)
|\
| PO4
|
Methylglyoxal
|
| F420-H2
|/
| Methylglyoxal reductase (unidentified) (PMID 18363381)
|\
| F420
|
L-lactaldehyde
|
| NAD
|/
| L-lactaldehyde dehydrogenase (NAD) (MJ1411) (PMID 16585745)
|\
| NADH
|
L-lactate
|
| GTP
|/
| CofB
|\
| GDP
|
2-phospho-L-lactate
|
| GTP
|/
| CofC
|\
| PPi
|
Lactyl (2) diphospho-(5')guanosine = LPPG
|
| 7,8-didemethyl-8-hydroxy-5-deazariboflavin (= FO) (see below)
|/
| Lactyl (2) diphospho-(5')guanosine:7,8-didemethyl-8-hydroxy-5-deazariboflavin 2-phospho-L-lactate transferase (CofD)
|\
| GMP
|
Coenzyme F420 = Coenzyme F420-0
|
| glutamate + GTP
|/
| Coenzyme F420-0:L-glutamate ligase (CofE)
|\
| GDP + Pi
|
Coenzyme F420-1
|
| glutamate + GTP
|/
| Coenzyme F420-1:L-glutamate ligase (CofE)
|\
| GDP + Pi
|
Coenzyme F420-2
|
| glutamate + ATP (or GTP)
|/
| Coenzyme gamma-F420-2:L-glutamate ligase (CofF)
|\
| ADP (or GDP) + Pi
|
Coenzyme F420-3


Graham, D. E., Xu, H., and White, R. H. 2003.
Identification of the 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase required for coenzyme F(420) biosynthesis.
Arch. Microbiol. 180:455-64.
PMID: 14593448

5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione (from riboflavin biosynthesis)
|
| 4-hydroxyphenylpyruvate (HPP) (from tyrosine biosynthesis)
|/
| FO synthase (CofG + CofH)
|\
| oxalate + NH3
|
7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO)



Paralogs included to help with resolution:

7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase-related protein (cluster A)
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase-related protein (cluster B)