Subsystem: Macromolecular synthesis operon
This subsystem's description is:
The macromolecular synthesis (MMS) operon contains three essential genes (rpsU, dnaG, rpoD) whose products ribosomal protein S21 (rpsU), DNA primase (dnaG), and the 70-kDal sigma subunit of RNA are necessary for the initiation of protein, DNA, and RNA synthesis respectively." (PMID: 8316085 Versalovic et al)
Originally observed in Escherichia coli and Salmonella typhimurium (PMID: 3005129 Erickson, et al) it is present in a variety of organisms.
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| Diagram | Functional Roles | Subsystem Spreadsheet | Description | Additional Notes | |||||||||
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The macromolecular synthesis (MMS) operon contains three essential genes (rpsU, dnaG, rpoD) whose products ribosomal protein S21 (rpsU), DNA primase (dnaG), and the 70-kDal sigma subunit of RNA are necessary for the initiation of protein, DNA, and RNA synthesis respectively." (PMID: 8316085 Versalovic et al) Originally observed in Escherichia coli and Salmonella typhimurium (PMID: 3005129 Erickson, et al) it is present in a variety of organisms. This SS was started by Ross Overbeek. Mol Microbiol. 1993 Apr;8(2):343-55.Links Conservation and evolution of the rpsU-dnaG-rpoD macromolecular synthesis operon in bacteria. Versalovic J, Koeuth T, Britton R, Geszvain K, Lupski JR. The macromolecular synthesis (MMS) operon contains three essential genes (rpsU, dnaG, rpoD) whose products (S21, primase, sigma-70) are necessary for the initiation of protein, DNA, and RNA synthesis respectively. PCR amplifications with primers complementary to conserved regions within these three genes, and subsequent DNA sequencing of rpsU-dnaG PCR products, demonstrate that the three genes appear to be contiguous in 11 different Gram-negative species. Within the Gram-negative enteric bacterial lineage, the S21 amino acid sequence is absolutely conserved in 10 species examined. The putative nuteq antiterminator sequence in rpsU consists of two motifs, boxA and boxB, conserved in primary sequence and secondary structure. The terminator sequence, T1, located between rpsU and dnaG is conserved at 31 positions in nine enterobacterial species, suggesting the importance of primary sequence in addition to secondary structure for transcription termination. The intergenic region between rpsU and dnaG varies in size owing to the presence or absence of the Enterobacterial Repetitive Intergenic Consensus (ERIC) DNA element. The rpoD gene contains rearrangements involving a divergent sequence, although two carboxy-terminal regions which encode functional domains are conserved in primary sequence and spacing. Our data suggest that primary sequence divergence and DNA rearrangements in both coding and non-coding sequences account for the interspecies variation in operon structure. However, MMS operon gene organization and cis-acting regulatory sequences appear to be conserved in diverse bacteria. PMID: 8316085 | |||||||||||||
